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KMID : 0613820090190101478
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Journal of Life Science
2009 Volume.19 No. 10 p.1478 ~ p.1483
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Solid Cultivation of Fibrinolytic Enzyme (Bacillokinase) from Bacilis subtilis BK-17
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Jeong Yong-Kee
Beak Hyun
Seo Min-Jeong
Kim Min-Jeong
Lee Hye-Hyeon
Joo Woo-Hong
Kim Jung-In
Choi Young-Hyun
Chung Kyung-Tae
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Abstract
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A solid-state culture based on grain materials was attempted to produce a fibrinolytic enzyme for blood circulation improvement using Bacillus subtilis BK-17. The spore, rather than vegetative cell inoculation, of B. subtilis BK-17 on the solid-state culture was effective in the production of a fibrinolytic enzyme. Maximum spore production was obtained with a SFM medium (0.8% nutrient broth, 0.05% yeast extract, 10?¹ M MgCl₂, 10?³ M FeCl₃, 10?⁴ MnCl₂, 10?? M dipicolic acid, pH 6.5). Optimal pH and temperature were pH 6 and 30℃, respectively. The spore production reached a maximum at 60 hours of incubation. Bacillus subtilis BK-17 on the mung bean solid-state culture produced greater fibrinolytic activity, and less activity was seen in other grains such as kidney bean, soybean and corn. Protein and lipid contents of fermented soybeans were about 10 - 30% more than those of unfermented soybeans. Amino acid content was also 5 - 20% more than that of unfermented soybeans. These results indicated that fermented solid-state culture medium, fermented soybean in this case, can be utilized as a food supplement.
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KEYWORD
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Bacillus subtilis, fibrinolytic enzyme, solid culture, fibrin, fermented soybean
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